Final Observation

My final observation took place on November 19, 2012. There was a noticeable difference in the MicroAquarium. I found no 'large' organisms that had survived since the last observation. There were many diatom shells mostly along the bottom but they were all throughout the tank as well. After surveying the habitat it seemed that the only remaining organisms were those who survived on detritus. Vorticella were still prevalent along with many other single celled organisms such as Litonotus. The amoeba, centropyxis sp, was observed again along with other amoeba specimens. It looks as though the food chain is collapsing from the 'top, down'. Larger organisms with relatively higher levels of need have dwindled to what appeared to be zero while single celled organisms seemed almost unaffected.

Observation 3

My third observation took place on November 13th. My aquarium seemed pretty lively, probably equal to last time. I saw three different types of amoeba wandering around in different areas, a centropyxis, difflugia,and a saccamoeba. The saccamoeba was the only one that wasn't moving around. The others were actively moving about looking for food I would imagine.

There were not many large organisms this time. I imagine with the food pellet running low that the food chain is collapsing to the simpler organisms that can live on detritus. Many paramecium and cyclidium were spotted darting around but they were too quick to capture a good image. I did find a 'swarm' of what were thought to be litonotus. They were moving much too quickly to capture an image of an individual so I just took an image of the entire group.

Observation 2

After my initial observation on Friday, October 26, a food pellet was put into my MicroAquarium. The food pellet was an "Atison's Beta Food" pellet manufactured by Ocean Nutrition. The ingredients analysis were listed as follows; Fish meal, wheat flower, soy meal, krill meal, minerals, vitamins and preservatives. Analysis: Crude Protein 36%; Crude fat 4.5%; Crude Fiber 3.5%; Moisture 8% and Ash 15%. 

My last observation was on November 6th. Again, I noticed first that the water level had dropped considerably. Once the aquarium was under the microscope I noticed an increased level of activity compared to last time. There were many very small organisms moving around in the water. Some of them were difficult to track and get a good picture of. I managed to get a picture of a Paramecium as it was wandering around. It was recognizable by the groove which runs down the center and aids in feeding, it is visible in the picture if you look closely.

A Litonotus was also seen making its way through the water. It was quite a bit harder to catch and get a good photo. Luckily the the picture I got made it fairly easy to identify by its distinctive shape.

Many Vorticella were seen throughout the aquarium. This time they seemed much more lively than previously. The majority of observed specimens extended and and all had active cilia. When viewing them closely I could observe a whirlpool effect as water and detritus were moved by the cilia. 

Rotifers were plentiful and were active in pretty much every part of the aquarium. They were mostly seen eating plant matter or swimming towards a new place to feed. In the two pictures that follow you can see a Rotifer approaching a plant and then proceed to feed on it in the second picture.

A Rotifer swimming towards its next meal.

The same Rotifer feeding.

Finally I observed an organism that was quite odd looking and larger than anything else I have mentioned. This organism was refered to as a Cyclops and it resembled shrimp or similar crustacean. It reminded me of a crawfish as well. It was easy to identify due to its shape and prominent red eyes.

The next picture is a little blurry but shows the basic shape of the specimen well.

Hopefully next observation my aquarium will have matured more and there will be more strange creatures for me to discover.

First Observation

On Friday, October 26th, I made my first observation of the MicroAquarium since we put them together in class. The first thing I noticed was that the water level had visibly gone down. At first glance it appeared as if the previously unidentified bug and plants were still alive.

Figure 1. The water level had dropped quite a bit since the MicroAquarium was constructed.

Luckily the lab was not very busy and I immediately had access to a microscope. The microscope at my particular station was a Leitz Laborlux 11 with an attached camcorder and remote for taking still images through the microscope. What seemed to be a pretty quiet  and still aquarium was suddenly very busy looking under magnification. Some organisms were darting around quickly and getting a picture of them would prove to be particulaly tough. I noticed many Vorticella (figure 2) throughout the water (Patterson 1996). Some were alone and some were in small groups. They were easy to find because they are attached to the plants with a recoil-able stalk.

Figure 2. Vorticella specimens were common throughout the MicroAquarium.

As I continued observing the aquarium I was also able to see many examples of Rotifer Euchlanis (figure 3, figure 4) swimming around (Patterson 1996). They were never too far from plant life and were observed floating detritus in the aquarium water. They are fairly quick swimmers but would only do so in short bursts. 

Figure 3. A stationary Rotifer Euchlanis sp 

Figure 4. A Rotifer Euchlanis sp swimming.

 While scanning the water I noticed something that looked like a ball or a sphere. On further investigation I could see that it was covered in spines. It was motionless so I took advantage to take a good picture (figure 5). The organism was identified as Acanthocystis sp (Patterson 1996). They are planktonic unicellular organisms and were found to be common in the water. I also happened to find a water flea (Cyclidium sp) nearby on a plant (figure 6). I had seen many of them as I scanned the aquarium but they were usually moving much to fast to get a good picture.

Figure 5. Acanthocystis sp with visible spines.

Figure 6. Cladocera sp, also known as a Water Flea.

Many diatoms were also visible floating in the water. There were also many ciliates and flagellates swimming through the water. They were much too small and fast for me to coordinate a good picture at this particular station. This observation went very well although I wish I could have gotten some more pictures. I was happy to see my MicroAquarium was very lively and all seemed well in it's little environment.

MicroAquarium Set-up

Last Wednesday, I set up my MicroAquarium in botany lab as the first step in my term project. I chose to use water from Meads Quarry for my aquarium. This area, located at N35 57.162 W83 51.960 880, is a rock quarry with partial shade exposure (McFarland 2012). Since Meads Quarry was previously an active quarry, many minerals have been exposed and likely made their way into the water. I hope this leads to some interesting observations/results throughout the project. Sediment from Meads Quarry was also included and settled at the bottom of the aquarium. I also happened to see a bug darting around in the sample container and managed to capture and put it inside the aquarium as well. 

Next, I installed some plant life within the aquarium. I put a total of 3 plants in the aquarium, 2 Amblestegium sp and 1 Utricularia gibba L (McFarland 2012). I then properly labeled my aquarium so I could easily keep track of it. Once everything was set up nicely I took some time to look at my aquarium through the microscope. I saw many small organisms darting around. Some were swimming or floating around in circles while others were making fairly direct lines to their destination. The organism floating in circles was identified to likely be a diatom by my TA, Marisol. These organisms are single-celled and unique in the fact that they have silica based 'shells'. Over time, the discarded shells of dead diatoms will eventually become silicate sediment. 

I hope to view many more interesting organisms and activity in the coming weeks. Below is a picture of the MicroAquarium on Day 0.

Bibliography/Works Cited

Cook R, McFarland K. 2013. General Botany 111 Laboratory Manual. 14th ed, Knoxville (TN).

McFarland K. Botany 111 Fall 2012. [Internet]. 2012. Available from :http://botany1112012.blogspot.com/

Patterson, D.J. 1996 Free Living Freshwater Protozoa Washington D.C. Manson Publishing